J.Jpn. Surg. Soc.. 96(10): 718-723, 1995
Original article
CALCIUM METABOLISM IN PORCINE LIVER TRANSPLANTATION
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First Department of Surgery, Hokkaido Univercity School of Medicine, Sapporo, Japan Jun Kimura, Yasuaki Nakajima, Takashi Omura, Motoshi Tamura, Koji Ito, Hideya Isai, Junichi Uchino |
Group I pigs were added no warm ischemia (n=6). In group II, donors were killed by KCI, and the livers were Ieft in situ for 60 min (n=6). After anastomosing the suprahepatic vena cava, revascularization was carried out employing PA. Initial reperfused blood was collected through the infrahepatic vena cava.
All pigs survived more than 4 days in group I, whereas all died within 2 days in group II. In group II, Ca2+ levels of initial reperfused blood were significantly lower than those of arterial blood. Ca2+ levels of initial reperfused blood in group II were significantly lower than those in group I. Within 90 min after revascularization, biliary output and biliary calcium content and excretion in group I recovered to 30%, 100% and 40% of normal, respectively, but in group II only 2 animals could excrete bile.
In conclusion, in the liver with 60 min warm ischemia, Ca2+ influx occurred immediately after revascularization followed by decreased calcium excretion in bile, which suggested that accumulation of calcium might be one of the causes of warm ischemic injury.
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