J.Jpn. Surg. Soc.. 95(4): 234-241, 1994

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Original article

HISTOLOGIC AND CELL KINETIC ALTERATION OF GASTRIC MUCOSA UNDER CONTINUOUS DUODENAL REGURGITATION IN EXPERIMENTAL DOG MODEL

The experimental models for duodenal regurgitation were established using Schimilinisky-McCann's procedure in dogs. Histology, cell kinetics, and degree of DNA methylation in the gastric mucosa of those models were examined at 3, 6, and 12 month periods after the procedure and compared with those in normal mucosa. Histologically, minor changes of gastric mucosa such as superficial gastritis and erosion, were observed at the 3 month period, whereas hyperplasia and atrophy of the mucosa were observed at the 6 and 12 month periods, respectively. Cell kinetic analysis using bromodeoxyuridine (BrdU) and anti-BrdU antibodies revealed a significantly high labeling index (L.I.) in the hyperplastic mucosa and also revealed a tendency of high L.I. in the atrophic mucosa at the 6 month period in comparison with that in normal mucosa. A high level of DNA methylation was identified in the mucosa that showed a high L.I. of BrdU at the 6 and 12 rnonth periods. These results suggest that hyperplastic and subsequent atrophic changes of the gastric mucosa under continuous duodenal regurgitation are closely related with rapid cell proliferation and a high level of DNA methylation, and that the duodenal juice could play a crucial role in the carcinogenesis of the stomach.

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