J.Jpn. Surg. Soc.. 94(5): 516-525, 1993

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Original article

INFLUENCE OF HOMOLOGOUS SERUM ON ENDOTHELIAL CELL GROWTH FACTOR WITHDRAWAL

In examined the influence of endothelial cell growth factor(ECGF)withdrawal upon the growth and PGI₂ production of human endothelial cells (HECs) maintained in either fetal bovine serum (FBS) or human serum (HS).
For the study of growth, enzymatically harvested cells were passed twice, divided into seven groups and maintained in medium containing 20% FBS with ECGF (0, 0.1, 1, 10, 100μg/ml) or 20% HS with ECGF (0, 100μg/ml) (n=8).Growth curve was generated from daily cell counts for ten days. Cells were dead in medium containing FBS with ECGF in concentration of 1μg/ml or less, however, cells were sustained and grew in HS without ECGF.
For the study of PGI₂ production, harvested HECs were divided into 2 groups and cultured in medium containing 20% FBS or HS (ECGF: 100μg/ml) (n=12). Second passage cells were divided into 3 groups respectively and cultured in different ECGF concentration (0, 1, 100μg/ml) for 48 hours. Cells were stimulated with thrombin (2U/ml) and PGI₂ production was measured by radioimmunoassay. Cells cultured in medium containing HS produced more amount of PGI₂ than in FBS in any concentration of BCGF (19±11pg/10⁵ cells in FBS v.s.89±80pg/105 cells in HS:p＜0.05, ECGF:100μg/ml)
Second passage HECs cultured in mediunl containing HS were viable and maintained the ability of PGI₂ production even without ECGF.

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