J.Jpn. Surg. Soc.. 94(5): 480-493, 1993

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Original article

PANCERATIC REGENERATION AFTER MAJOR PANCREATECTOMY AND EFFECT OF INSULIN ADMINISTRATION IN DOGS

Regeneration of the remnant pancreas after more than 92% pancreatectomy with or without insulin treatment was studied in dogs. All dogs developed diabetes mellitus (DM) immediately after surgery. For the dogs in insulin-treated group, porcine insulin (NPH) was injected subcutaneously daily to maintain the fasting blood sugar levels from 70 to 130mg/dl. All dogs without insulin treatment died within 7 weeks after surgery, whereas 87.5% of the dogs receiving insulin treatment survived until 12 weeks, and 57.1% of surviving dogs were free of DM. DNA and RNA synthesis and ornithine decarboxylase (ODC) activity in the remnant pancreatic tissue 3 days after surgery were significantly greater in the insulin-treated dogs than in the untreated dogs. Endogenous insulin secretion, exocrine pancreatic function and the regeneration rate of the remnant pancreas at the 6-7th week were significantly better in the insulin-treated dogs. Furthermore, the regeneration rate at the 6-7th week was highly correlated with DNA and RNA synthesis and ODC activity in the remnant pancreas tissue 3 days after surgery. After major pancreatectomy in dogs, insulin treatment enhances the proliferation of the remnant pancreas during the early period and maintains endogenous insulin secretion for a long period, prolonging survival and promoting pancreatic regeneratlon.

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