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Abstract]
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Full Text PDF] （in Japanese / 521KB）
[Members Only And Two Factor Auth.] J.Jpn. Surg. Soc.. 93(11): 1372-1377, 1992
Original article
INDUCTION OF IMMUNOGENIC VARIANT OF A MURIN FIBROSARCOMA
Immunogenic variant was induced from the methylcholanthren induced fibrosarcoma（MCA-F）by in vitro treatment with the mutagene 1-methyl-3-nitro-1-nitrosoguanidine（MNNG）. D-10 tumor cell which was cloned from MNNG treated MCA-F tumor cell was rejected by normal syngeneic C3H-HeJ mice but not by 650 rad irradiated immunosuppressed mice. Host which had rejected D-10 tumor growth, rejected parental MCA-F tumor cells. But active immunotherapy using D-10 tumor cell against MCA-F tumor cells. But active immunotherapy using D-10 tumor cell against MCA-F tumor bearing mice was not successful. Cytotoxic T cell（CTL）established from D-10 immunized spleen cells showed D-10 specific cytotoxic activity and not lysed parent MCA-F cells. CTL clone C-E-6 showed specific cytotoxic and proliferative activity against D-10 cell. In vivo tumor neutralysing assay also showed its specificity against D-10 tumor cell.
Active immunothrapy and adoptive immunotherapy using immunogenic variant, D-10 was not successful. D-10 tumor cell possesses very strong neoantigen induced by MNNG treatment and parental MCA-F antigen.
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