J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 2056KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 93(9): 960-963, 1992

Report on the annual meeting

A NEW METHOD OF IN VITRO CHEMOSENSITIVITY TEST USING MULTICELLULAR SPHEROIDS OF CHOLANGIOCARCINOMA CELL LINE COCULTURED WITH FIBROBLASTS

1) Departments of Surgery, St. Marianna University School of Medicine, Kawasaki, Japan
3) Departments of Pathology, St. Marianna University School of Medicine, Kawasaki, Japan
2) Japan Research Center, W.R. Grace Co., Tokyo, Japan

Sunao Kubota1), Toshiaki Takezawa2), Yuichi Mori2), Toshifumi Takakuwa3)

We applied the multicellular spheroids which consist of cholangiocarcinoma cell line (MEC) and human dermal fibroblasts (HDF) to in vitro chemosensitivity test. Five-day multicellular spheroids were incubated with 1.5μg/ml of mitomycin C (MMC) for 24 hrs. Then, cell kinetics of MEC and HDF in a spheroid was determined by flow cytometric analysis. Twenty four hrs after treatment with MMC, both MEC and HDF were accumulated on S phase. Seven-day after treatment, DNA histogram in MEC returned to normal, but that of HDF was disappeared. These results showed that the multicellular assay could be more like on in vivo like chemosensitivity test.

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