[
Abstract]
[
Full Text PDF] （in Japanese / 417KB）
[Members Only And Two Factor Auth.] J.Jpn. Surg. Soc.. 93(4): 388-392, 1992
Original article
PROLIFERATIVE ACTIVITY IN GASTRIC CANCER WITH Ki-67 AND PROPIDIUM IODIDE:
ANALYSIS BY FLOW CYTOMETRY
Growth fractions in the cell cycle were demonstrated by flow cytornetry with monoclonal antibody Ki-67 for gastric cancer. By setting the cut off line at the lowest channel number of S-phase, the Ki-67 labeling rate was calculated by Ki-DNA dual fluorescence analysis. In addition to 32 gastric cancers, we examined three cell lines (Colo 320, NUGC4 and MKN28) and compared the result with the BrdUrd labeling rate. The G
0G
1 ratio obtained with BrdUrd was generally correlated with the G
0+G
1 ratio obtained with Ki-67. The S-phase fractions obtained with Ki-67, however were a little different from those obtained with BrdUrd because of the existence of S
0. The mean Ki-671abeling rate of gastric cancer was 45.1% (16.2-66.3%). Fifteen cases received Ki-67 immunohistochemical study in the same samples. The results of flow cytometric analysis were parallel to those of microscopic study, and a correlation line: y=0.6261x +15.9145, r=0.8031 (p＜0.001) was obtained. Ki-67 antibodies may provide useful information on cell kinetics.
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