J.Jpn. Surg. Soc.. 91(9): 1417-1420, 1990
Report on the annual meeting
GRANULOCYTES CAUSE REPERFUSION INJURY AFTER 180 MINUTE HYPOTHERMIC CARDIOPLEGIC ARREST IN DOGS
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Department of Surgery, Keio University, Tokyo, Japan H. Iseki, Y. Soma, K. Onoguchi, H. Shimizu, T. Inoue |
Method: Canine hearts were arrested with modified St. Thomas cardioplegia, and kept for 180 min. at the temperature of 15℃. Hearts were reperfused with leukocyte depleted, filtor(+), or whole blood, filtor(-). Parameters were such: %LV Emax (Emax after reperfusion vs. that of pre-ischemia), CPK-MB, Peroxidation products, Myocardial water content rate, and coroary blood flow rate.
Results: The filter(+) group showed better recovery of left ventricle function (%Emax) especially after 2 hrs. Reperfusion injury was less in the filter(+) group from CPK-MB viewpoint. The increase of peroxidation products shows free radical generation in the filter(-) group, which means the products of free radicals by leukocyte. Coronary blood flow rate was higher in the filter(-) group than in the filter(+) group till 30 min. after reperfusion, but the difference disappeared after 30 min., which means elongation of hyperemia in the filter(-) group. Myocardial water content didn't show any significant differences between the two groups.
Conclution: 1) Leukocyte rich blood attenuates reperfusion injury after 3 hrs. hypothermic cardioplegic arrest in dogs. 2) This experiment suggests that leukocyte produces free radicals. 3) Leukocyte depletion of reperfusate is an effective preventive measure of post ischemic reperfusion injury.
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