J.Jpn. Surg. Soc.. 91(9): 1264-1267, 1990

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Report on the annual meeting

PROGRESS IN SURGICAL ONCOLOGY

By using anti NHK-ras, anti K-ras, anti c-myc and anti EGF monoclonal antibodies, paraffin-embeded tissue specimens of gastric cancer, colorectal cancer and pancreatic cancer were immunohistologically studied. The incidence of expression of these oncogenes or growth factor was analyzed with statistical studies of survival rates and correlation to the tumor stage. Secondly, by using polymerase chain reaction, paraffin-embeded specimens of the periampullary carcinoma were confirmed the point mutation in Kirstein ras codon 12.
Frequency of expression of the oncogenes and growth factor was correlated to the stage of gastric cancer and colorectal cancer. In regard to survival rates, positive cases with gastric cancer for Kirsten ras or EGF were associated with significantly lower than negative cases. Regarding colorectal cancer, expression of Kirsten ras or c-myc could poor prognosis. Extremly high incidence of Kirsten ras mutation was shown in the pancreatic cancer. Point mutation was restrict to Kirsten ras codon 12 in pancreafu cancer. The highest mode of point mutation was GGT to GAT coded aspertic acid. Positive cases for mutation of Kirsten ras codon 12 in periampullary carcinoma were reviewed the specimens. Then one case of duodenal and ampullary carcinoma and two cases of distal bile duct carcinoma were revised the histological diagnosis to the pancreatic cancer. Detection of mutation of Kirsten ras codon 12 might distinguish the pancreatic cancer from periampullary carcinoma.

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