J.Jpn. Surg. Soc.. 90(11): 1848-1854, 1989

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Original article

FLOW CYTOMETRIC CELL CYCLE ANALYSIS USING A MONOCLONAL ANTIBODY TO BROMODEOXYURIDINE ON GASTRIC CANCERS

Flow cytometric cell cycle analysis using a monoclonal antibody to Bromodeoxyuridine was performed on 117 gastric cancers. Dissociated cells were stained with indirect immunostaining for BrdU (FITC-BrdU) and propidium iodide. Bivariate BrdU/DNA distribution were obtained using EPICS-C flow cytometry. Tumor ploidy was classfied as follows, D1: diploidy, D2: diploidy+aneuploidy, A1: single aneuploidy, A2 : multiple aneuploidies.
The ploidies of noncancerous gastric mucosas were all diploidy and thats of S-phase fraction (SPF) were ranged from 0.0% to 1.2%. In 117 gastric cancers, aneuploidy was observed in 80 cases, D2 : 38, A1 : 15, A2 : 27. SPF was higher in aneuploidy (14.5±5.1%) than diploidy (6.1±5.1%). Significant differences were observed between thats of D1 (6.1±2.5) and D2 (13.6±6.2), A1 (12.5±4.5), A2 (16.0± 3.2), and A1 and A2 (p＜0.01).
The patients with aneuploid tumors had poor prognosis than diploid tumors (p＜0.05). In concerned with DNA ploidy pattern, the patients with A2 had most poor prognosis than the other (p＜0.05). Furthermore, the patients with SPF over 10% had poor prognosis than that of SPF below 10%.
These results indicated that DNA ploidy pattern and SPF may possibly be useful prognostic markers for gastric cancers.

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