J.Jpn. Surg. Soc.. 90(10): 1799-1805, 1989
Original article
AN EXPERIMENTAL STUDY OF TISSUE INJURY ASSOCIATED WITH REPERFUSION IN ISCHEMIC LIMBS
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First Department of Surgery, Nagoya University School of Medicine, Nagoya, Japan Teruo Ikezawa |
In group II, serum CPK and LPO increased after reperfusion with peak levels of 38,000±9,800mU/ml and 20.4±3.7nmol/ml respectively, which were significantly higher than those in group I. (CPK: p＜0.02, LPO＜0.03)
In group III, the peak levels of serum CPK and LPO were regulated to the low level of 1,060±290mU/ ml and 9.2±4.5nmol/ml, respectively, which were significantly lower than those in group II. (CPK: p＜ 0.02,LPO＜0.04)
Additinal 13 dogs were divided into two groups in order to assess tissue LPO in the limb, liver, and kidney. Group A: control (n=5), group B:reperfusion after 6 hours of ischemia (n=8).
Tissue LPO level of 1.89±0.74nmol/mg-protein in the gastrocnemius muscle in group B was significantly higher than that in group A (p＜0.02), although there was no significant difference in the gracilis muscle, liver, and kidney.
These results prove indirectly the participation of lipidperoxidative reaction by active oxygen in the mechanism of development of reperfusion injury, and suggest the preventive effect of alpha-tocopherol to reperfusion injury.
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