J.Jpn. Surg. Soc.. 90(4): 580-585, 1989

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Original article

EFFECTS OF EXOGENOUS INSULIN ON OXIDATION OF GLUCOSE AND FATTY ACID IN SEPTIC RATS

The effects of exogenous insulin on oxidation of glucose and fatty acid were investigated in septic (n=20) and control rats (n=20). Sepsis was induced by ligation and puncture of the caecum. The rats received intravenous nutrition with glucose as a non-protein calorie for 27 hours. Ten rats in each group received insulin intravenously at a rate of 0.64U/kg/hr during the last 6 hours of the intravenous nutrition, U-¹⁴C-glucose or 1-¹⁴C-linoleic acid at a dose of 1.563µ Ci each was injected as a bolus at the 21st hour of the intravenous nutrition. Cumulative ¹⁴CO₂ production was measured for 6 hours after the injection of the radioactive substrates. ¹⁴CO₂ production from both glucose and linoleic acid was inhibited by the sepsis. ¹⁴CO₂ production from glucose was acceletated by exogenous insulin in the control rats, while it was not accelerated in the septic rats. Exogenous insulin did not affect ¹⁴CO₂ production from linoleic acid in both the control and the septic rats. These results indicate that, under the condition of sepsis, lowering blood surgar level with exogenous insulin does not reflect an increase in oxidatin of glucose.

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