J.Jpn. Surg. Soc.. 90(2): 168-180, 1989
Original article
BIOLOGICAL ACTIVITY OF HEPATOCELLULAR CARCINOMA BY ANALYSING NUCLEAR DNA PLOIDY PATTERNS AND USING ANTI BrdU MONOCLONAL ANTIBODY
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First Department of Surgery, Osaka City University Medical School, Osaka, Japan Tsai Jung-Rou |
These ploidy pattems were compared with histopathological and/or immunohistochemical studies of HCC using antibromodeoxyuridine monoclonal antibody.
The results are summarized as follows.
HCC of rats induced by DEN was occurred in 74 (65.5%) of 113 rats. Intravascular invasion of the Iivers were seen in 31 of 74 (41.9%) . Metastasis was in 25 of 74 (33.8%) .
The ploidy patterns of HCC in rats were classified into three types (types I, II and III) . These ploidy patterns were closely connected with histological types of HCC in Edmondson's classification, and in order of types changing from type I to type III,6c over polyploid cells, mitotic indices (MI) and labeling indices (LI) had a tendency to increase.
In human HCC, cell nuclear DNA ploidy patterns were classified into three types similary to experimental HCC. These ploidy patterns were closely related to histological types in Edmondson's classification. MI and 6c over polyploid cells were tendency to increase similary to HCC of experimental rats.
From the above results, measurement of DNA ploidy patterns and LI are considered a valuable paramenter in examining the biological activity of HCC.
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