J.Jpn. Surg. Soc.. 88(9): 1260-1263, 1987

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Report on the annual meeting

THE APPLICATION OF CO₂ LASER TO VASCULAR AND TRACHEAL RECONSTRUCTION ON RATS―HISTOLOGICAL PROCESS OF HEALING

CO₂-LASER assisted vascular and tracheal reconstruction are now feasible in rats. We observed the histological process of healing of the anastomosed sites. Ten end-to-side anastomoses between left renal vein and IVC, and another ten end-to-end anastomoses of transected IVC were carried out using low output CO₂ LASER. LASER was also applied to the reconstruction of transected trachea in 5 rats.
The patency of LASER-assisted anastomoses of end-to-side vascular reconstruction was excellent (100％) compared with that of conventional hand-sawn group (60％). Also, in end-to-end anastomoses, LASER produced 100％ of patency while hand-sawn technique yielded only 80％ of patency. Five tracheae reconstructed by LASER were all patent 1-4 weeks after anastomosis.
Histologically, intimal or epithelial lining of anastomosed site was almost completed within 7 days in LASER group. Four weeks after reconstruction, there was less scar, less hyperplasia of intima or epithelium than in hand-sawn group. Refiecting these findings, vascular compliance of anastomosed site was revealed to be more physiological in LASER group than in hand-sawn group.
In conclusion, vascular and tracheal reconstruction using CO₂ LASER seems to have many advantages over the conventional suture technique.

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