J.Jpn. Surg. Soc.. 88(9): 1256-1259, 1987
Report on the annual meeting
WOUND HEALING UNDER THE CONDITION OF MICROCIRCULATORY IMPAIRMENT ASSESSED BY THE FLUORESCEIN FLUORESCENCE METHOD
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Department of Surgery, Fujita-Gakuen Health University, Toyoake, Japan Hiroshi Amano, Takahiko Funabiki, Masahiro Ochiai, Katsumi Sugiue, Shinji Fujita, Hisashi Yamaguchi, Hisatomo Futawatari |
Ischemic segments were produced in rats by ligating a 6cm ileal loop, and removing the ligature after 4, 8 or 12 hours of occlusion. Post-ischemic microcirculatory impairment was assayed by two independent techniques. The gut segments were perfused intraarterially with a silicon elastomer after release of occlusion and microvascular patency was assessed by “blinded” scoring of a filling index based on the level of vessels filled. Microcirculatory reperfusion was evaluated by the score of the ultraviolet fluorescence pattern seen following i.v. injection of fluorescein. Intestinal healing rate was determined histologically at 2 to 7 days later. Fluorescence score, filling index and intestinal healing rate, all these showed significant correlation with duration of ischemia. These data suggest that microvascular patency and reperfusion may play an important role in intestinal healing from ischemic injury.
Based on these observations, 19 human cases of ischemic intestine were assayed by the fluorescein fluorescence method. It is concluded that the ultraviolet fluorescence pattern is a good clinical guide for the prediction of intestinal recovery after ischemic injury.
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