J.Jpn. Surg. Soc.. 88(8): 928-938, 1987

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Original article

CELL-MEDIATED IMMUNE RESPONSES OF PATIENTS WITH GASTROINTESTINAL CANCER TO ALLOGENIC TUMOR-ASSOCIATED ANTIGENS

Soluble tumor extract was prepared from cancer tissue of 172 patients with gastrointestinal cancer by the methods of hypertonic salt extraction (HSE) and 3M-KCL salt extraction (3M-KCL). Lymphocyte proliferative (LP) response and skin test was assessed with these tumor extracts. The LP response of cancer patients could be induced on 4 day-incubation in 10μg•protein/ ml of the extracts. The allogenic tumor extract induced the LP response with a close correlation to an autologous extract (p＜ 0.001). Both HSE and 3M-KCL extracts induced a positive LP response as well as delayed hypersensitivity of the skin in 20 to 30% with a good correlation (p＜ 0.001). The clinical stage related closely to a responsibility of LP response and of skin test to the tumor extracts except in stage IV. Gel filtration with Sephadex G-200 revealed the 5th graduated fraction (M.W. ＜10,000) responsible for the activity of LP test. The LP response dependes on T-lymphocytes. Cellular immune response to tumor antigen of gastrointestinal cancer could be demonstrated by LP response and skin test with the tumor extract prepared by HSE and 3M-KCL methods. Thus, it was concluded that the LP response and skin test is a useful parameter of tumor-specific immune response.

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