J.Jpn. Surg. Soc.. 87(3): 254-265, 1986

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Original article

EXPERIMENTAL STUDIES ON THE UPTAKE OF ANTICANCER DRUGS INTO CANCER CELLS IN HYPERTHERMOCHEMOTHERAPY
－EFFECTS OF IN VITRO AND IN VIVO HEATING ON THE UPTAKE OF 5-FLUOROURACIL INTO EHRLICH CELLS－

In order to clarify the influence of hyperthermia on the uptake of anticancer drugs into cancer cells, ddy mice and ³H-labelled 5-fluorouracil (³H-5-FU) were used. The Ehrlich ascites cells (E-cells) in addition to ³H-5-FU were heated in vitro at different temperatures and the uptake of the 5-FU into E-cells were observed with the passage of time, while ³H-5-FU was given to an E-cell “planted mice, to which local and total-body hyperthermias were performed at different temperatures for the uptake of the 5-FU into the Ehrlich solid tumors to be measured at intervals. The uptakes of 5-FU into E-cells and the Ehrlich solid tumors was observed by ³H-5-FU uptake and autoradiography respectively. The results are summarized as follows:
1) There was a significant sthenia of the 5-FU uptake into E-cell, as it was heated in vitro at 43℃ for 30 minutes and 60 minutes. This result was confirmed by autoradiograph. The sthenia was only found in E-cells remarkably, and not in normal cells.
2) In a tumor bearing mouse, there was a significant sthenia of the 5-FU uptake into the Ehrlich solid tumors as it was heated at local hyperthermia (42℃) and total-body hyperthermia (42℃) for 30 minutes and 60 minutes. The result was confirmed by autoradiograph. The sthenia was only found in the Ehrlich solid tumors remarkably, and not in normal tissues.
This results show an antiplastic increment by hyperthermochemotherapy that anticancer drugs can be included into the cancer cells and the Ehrlich solid tumors more easily by heating.

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