J.Jpn. Surg. Soc.. 86(2): 139-147, 1985

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Original article

ENDOSCOPIC AND HISTOPATHOLOGICAL STUDIES OF THE EXPERIMENTAL ESOPHAGEAL CANCER IN THE BEAGLES

In order to obtain a reliable experimental model simulating human esophageal cancer, endoscopic and histopathological studies were undertaken in the esophageal cancer produced in the beagle dog. Thirty-seven dogs had been given a solution of N-Ethyl-N’-nitro-N-nitrosoguanidine (ENNG) at a concentration of 150μg/ml for 3ー9 months. Follow-up studies included serial endoscopy and biopsy, and almost all animals were eventually sacrificed for histological examination.
The results were as follows:
1) Squamous cell carcinoma was observed in 5 out of 22 female dogs, while none in male dogs at all.
2) For the induction of squamous cell carcinoma in the esophagus, administration in the condition of 150μg/ml (75mg/day) for 6ー9 months was most suitable.
3) Almost all of esophageal lesions were protruding and well-differentiated squamous cell carcinoma with invasion of the submucosa.
4) The stages of hyperplasia, dysplasia and squamous cell carcinoma in the esophagus were chronologically followed.
5) Carcinoma had been observed in the stomach about 4 months prior to the appearance of esophageal carcinoma.
6) This experimental model was proved to be useful for studies on histogenesis of human esophageal cancer both light and electron microscopically.

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