J.Jpn. Surg. Soc.. 85(8): 791-798, 1984

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Original article

LIVER REGENERATION AND THE IMMUNE SYSTEM
1. IN VITRO AND IN VIVO ACTIVATION OF LYMPHOCYTES BY LIVER REGENERATION

When syngeneic lymphocytes and mitomycin C (MMC) treated regenerating liver cells prepared from a partial hepatectomized mice are cultured together, the in vitro DNA synthetic response is activated (sML^HLR : syngeneic mixed hepatectomized liver cell-lymphocyte culture). Ia⁺ Kupffer cells play an important role as stimulators in the responses, since the stimulating activity of regenerating liver cells is lost by the pretreatment of them with anti-Ia monoclonal antibody plus complement or the removing Kupffer cells from them. The lymphocytes are activated also in vivo during liver regeneration after a partial hepatectomy. Because, when lymphocytes prepared from hepatectomized mice are cultured with regenerating liver cells, lymphocytes are stimulated to accelerate their DNA synthesis in a typical manner of the secondary immune responses (secondary sML^HLR). In primary sML^HLR, the responder cells are mainly Lyt-1⁺ whereas in secondary sML^HLR they are mainly Lyt-2⁺. The mechanism of changing the Lyt phenotype of major responder cells from Lyt-1 to Lyt-2 during sML^HLR is discussed.

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