J.Jpn. Surg. Soc.. 84(4): 301-309, 1983

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Original article

ANTIGENIC STRUCTURE OF NCA-2 FROM MECONIUM AND COMPARISON OF THIS ANTIGEN WITH NORMAL FECAL ANTIGEN FROM ADULT FECES AND CARCINOEMBRYONIC ANTIGEN FROM MALIGNANT TISSUES

Carcinoembryonic antigen (CEA)-related antigen were isolated from meconium and compared with those in adult feces and tumor tissues. NCA-2 and NCA preparations were isolated from meconium. By conventional anti-CEA antisera, NCA-2 from meconium, NFA-2 from adult feces and CEA from tumor tissues were indistinguishable from each other. However, the CEA-distinctive determinant was absent in NCA-2 molecules. The NFA-2 distinctive determinant was detectable in 20% on NCA-2 molecules.
The molecular weight of purified NCA-2 was estimated to be 150,000 to 170,000 determined by SDS-PAGE.
NCA-2 revealed β-region mobility electrophoretically. NCA-2 had amino acid and carbohydrate compositions similar to those of CEA and NFA-2.

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