J.Jpn. Surg. Soc.. 83(2): 175-185, 1982

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Original article

DISSOCIATION OF NATURAL KILLER ACTIVITY AND AFFINITY OF SUBPOPULATIONS OF PERIPHERAL LYMPHOCYTES IN CANCER PATIENTS AND NORMAL DONORS

I measured the affinity of lymphocytes to target cells that are sensitive to natural killing and compared it with natural killer activity. By culturing measled-virus infected HeLa cells (M-HeLa) until they grew confluently on the surface of Superbeads, I carried out mixed cultures of these cells mixed with various lymphocyte fractions labeled with ⁵¹Cr, and by isolating and counting the lymphocytes that adhered to M-HeLa cells, I calculated the affinity of each lymphocytes to the entire lymphocytes.
Both T and non-T lymphocytes were capable of mediating NK activity against cells of M-HeLa. When T cells were further fractionated into Tγ and non-Tγ cells, both fractions demonstrated NK activity. In healthy donors, non-T cells and Tγ cells were more active than T cells and non-Tγ cells. However, in cancer patients, NK activity of non-T cells was not higher than that of T cells.
The affinity of lympocytes to M-HeLa in cancer patients was lower than that in healthy donors, and the decrease in the affinity of cells was more marked than non-T cells.

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