J.Jpn. Surg. Soc.. 82(1): 12-21, 1981

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Original article

STUDIES ON INTRACADAVERIC ABDOMINAL ORGAN PERFUSION USING ARTIFICIAL BLOOD (FLUOSOL-DA)

In order to keep the cadaveric abdominal organs in good condition for transplantation, the organ perfusion with the perfusate containing artificial blood was studied using 24 mongrel dogs. Except where stated all perfusates were oxygenated by 100% oxygen.
The results obtained as follows:
1. Total systemic peripheral resistance (TSPR) of the dogs perfused with Fluosol-DA in room temperature, lower than that of the dogs perfused with lactate Ringer’s solusion in room temperature.
2. TSPR of the dogs perfused with Fluosol-DA in hypothermia, lower than that of the dogs perfused with Fluosol-DA in room temperature. When the dogs were perfused with Fluosol-DA in hypothermia added 1.5% CO₂, TSPR lower than that of the dogs perfused with Fluosol-DA in hypothermia without CO₂.
3. Also the addition of 1.5% CO₂ prevented PCO₂ decrease and pH increase during perfusion.
4. When the warm ischemic time was within 30 minutes, the transaminase (GOT, GPT) and lactate dehydrogenase (LDH) in the perfusate were stable. However, when the warm ischemic time exceeded 60 minutes, GOT, GPT, and LDH in the perfusate increased during the initial one hour.
5. There were no marked changes in electrolytes, blood urea nitrogen, and creatinine in the perfusate.

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