J.Jpn. Surg. Soc.. 81(9): 1068-1072, 1980
Report on the annual meeting
STUDIES OF TUMOR IMMUNOLOGY BY THE USE OF CULTURED CELL LINES DERIVED FROM HUMAN CARCINOMAS
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Department of Surgery, Institute of Medical Science, University of Tokyo Morimasa Sekiguchi |
1) A solubilized membrane antigen was extracted from the CKS cells derived from serous cystadenocarcinoma of the ovary. Nine out of 12 patients with ovarian carcinoma responded to this antigen in the leukocyte migration inhibition test (Clausen's method), whereas only 2 out of 22 controls did so (0/6 normal healthy females, 1/5 benign ovarian tumors, 1/6 cervical carcinomas, 0/2 uterine myomas, 0/3 gastro-intestinal carcinomas).
2) Another solubilized membrane antigen was prepared by the 3M KC1 extraction method from the KATO-II cells derived from singet ring cell carcinoma of the stomach. Eight out of 17 patients with gastric carcinoma exhibited leukocyte migration inhibition with this antigen, whereas 4 out of 16 controls did so.
3) Assay methods to estimate K-cell and NK-cell activities of the peripheral lymphocytes were established by means of microcytotoxicity utilizing the HMV cells of melanoma origin as nonspecific target cells. The majority of patients with gastric carcinoma showed an identical level of K-cell activity with that in normal healthy subjects, and 2 patients had impaired activity. The activity tended to lower in 1 week postoperatively and to reach the preoperative level in 1 month postoperatively.
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