J.Jpn. Surg. Soc.. 80(7): 611-626, 1979
Original article
EXPERIMENTAL STUDIES ON FREEZING PRESERVATION OF PANCREATIC ISLETS OF RAT
|
Department of Surgery, Cancer Research Institute Hospital, University of Kanazawa Susumu Ohno |
2) Pancreatic islets of each experimental groups were preserved from 7 to 10 days in deep refrigerator at -80°C or -196°C in a liquid nitrogen with Dulbecco modified Eagle's medium supplemented with10%-20% D.M.S.O. (dimethylsulfoxid), and the thawing was performed in 37°C water bath.
3) Pancreatic islets of each groups were isolated by collagenase digestion method.
4) Isolated pancreatic islets of group III refrigerated at -196 °C in the medium supplemented with 20% D.M.S.O. showed morphological resembling closely to normal isolated pancreatic islets (controlgroup) and was encountered approximately 51.3% insulin secretion as compared with control group.A 300 of pancreatic islet isolated and preserved with this condition was transplanted into the portal vein of Streptozotocin induced diabetic rats. As a result diabetic rats recovered from diabetic state and the levels of glucose in blood maintained in normal ranges for following 20 weeks after transplantation.
5) Many B-cells were found in the transplanted islets in liver, but B-cells of recipient pancreas showed marked decrease in number and degeneration in shape.
6) The period of time needed about 4 weeks until isolated pancreatic islets indicated normal function biologically after transplantation.
In conclusion, isolated pancreatic islets with 20% D.M.S.O. (-196℃ )of group III retained their biological function in vitro and in vivo. The transplantation of isolated pancreatic islets with experimental diabetic rats.
To read the PDF file you will need Adobe Reader installed on your computer.