J.Jpn. Surg. Soc.. 80(5): 466-481, 1979
Original article
INFLUENCE OF INTESTINAL FLORA ON BILE ACIDS METABOLISMS USING GERM-FREE MICE
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The First Department of Surgerry, Nippon Medical School (Director : Prof. Akiro Shirota) Shigeo HATTA |
1) The bacteria setted well in upper jejunum, in terminal ileum and in coecum of the mice of all 3 groups (group-E, -S and -ES).
2) Control experiments revealed that in GF total BA in intestinal contents was lower and that in intestinal contents was higher significantly than those in conventional mice (CV), respectively. In addition, while an appreciable amount of BA was to be free in CV.
An increasing of total BA in gall bladdar bile and a decreasing in intestinal contents were observed in group-E. On the other hand, a qulitative rather than quantitative change was observed in group-S, where atrend of increasing of free BA was indicated. Changed of BA in group-ES were similar to those in group-S during 1-3 wks after the administration of bacteria, and then resenbled those in group-E during the next 4 wks. After 9 wks, the amount and the character of BA in group-ES became very close to those in CV.
3) Three components of BA (cholic acid, 80% ; chenodexycholic acid, 1.4% ; ursodeoxycholic acid or hyodeoxycholic acid, trace) were identified both in gall bladdar bile and intestinal contents of GF. In group-E and group-S, no other components than above three were detected. In group-ES, however, free and conjugated 7-ketodeoxycholic acids in bile and free deoxycholic acid in intestinal contents were identified quite a little. This phenomenon seems to be the tendency to approach the components of BA in CV.
These results thus indicate that the alterations in the amount and in the constituent of BA may be elicited by the coexistence of E. coli and S. faecalis in intestine of mice.
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