J.Jpn. Surg. Soc.. 80(1): 20-31, 1979

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Original article

CELL-MEDIATED IMMUNITY AND ITS RELATIONSHIP TO AUTOLOGOUS SERUM IN TUMOR-BEARING MICE

In order to study on the development and persistence of tumor immunity during tumor growth, cell-mediated immunity and its relationship to autologous serum were ingestigated in DDS mice with tumor produced by subcutaneous transplantation of Ehrlich ascites tumor cells. Using the regional axillary lymph nodes as the source of lymphoid cells, the cell-mediated immunity was assessed by macrophage migration inhibition test and lymphocyte blastogenesis to Phytohemagglutinin (PHA) and also, examined in relation to the autologous serum which was separated from the blood obtained by cardiac puncture.
Activity of macrophage migration inhibition factor (MIF) was maximal 5 days after transplantation when tumor became palpable, then decreased, and disappeared completely 14 days after transplantation.The level of the lymphocyte blastogenesis to PHA remained unchanged for 10-14 days after transplantation, and then decreased with tumor growth.
On the other hand, the influence of the autologous serum on these cellular responses was observed to show the inhibitory effect which already presented 3 days after transplantation and increased with tumor growth.
These data indicated that cell-mediated immunity develops and persists in the early stage of the tumor growth but decreased thereafter and that, inhibitory effect of serum factors on the cell-mediated immunity is a suggestion for the progressive growth of tumor.

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