[
Abstract]
[
Full Text PDF] （in Japanese / 1435KB）
[Members Only And Two Factor Auth.] J.Jpn. Surg. Soc.. 79(11): 1397-1402, 1978
Original article
RENAL CRYOPRESERVATION (3). THE EFFECT OF FINAL TEMPERATURES ON SURVIVAL OF CANINE KIDNEYS FROZEN TO BELOW -22℃
The canine kidneys perfused with 800 ml of cryoperecipiteted plasma containing 12.5% DMSO were frozen at rates of 2.0 ° to 4.0℃/min to －40 ° , －50 ° , and －75℃, respectively. Six out of eight kidneys frozen to －40 ℃ had a quite noticeable urine output within minutes after reimplantation. Four kidneys of this group ceased producing urine and become edematous and infarcted due to venous thrombosis. Renal function was maintained in the remaining two kidneys. However, after contralateral nephrectomy, one survived ten days and the other 5 days. Marked tubular necrosis and inter stitial fibrosis and hemorrage were common findings in this group. None of the kidneys frozen to －50 ° and －75℃ were functional due to thermal runaway and marked regional temperature differences after thawing. Severe renal cell damage and significnat increases in kidney weight were observed in the kidneys frozen to －50 ° and －75 ℃. The results demonstrated that temperatures of below －50 ° C were critical and the renal cell damage was more severe in the kidneys frozen to below －50℃.
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