J.Jpn. Surg. Soc.. 79(6): 489-499, 1978
Original article
STUDIES ON TUMOR-S PECIFIC CELL -MEDIATED IMMUNITY IN CANCER PATIENTS USING A MIXED LYMPHOCYTETUMOR CELL CULTURE REACTION
PART I. ANALYSIS OF CONDITIONS OF THE CULTURE
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Department of Surgery, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima (Director: Prof. T. Hattori) Hiroshi Ikeda |
1) Positive blastogenic responses to autochthonous irradiated tumor cells were observed in 12 of 37 patients with solid tumors. On the other hand, no responses were induced in any of 32 selected patients with gastric diseases by autochthonous normal mucosa cells. In the latter group, the stimulation index never exceeded a value of 1.4.
2) The optimal response of peripheral lymphocytes to autochthonous tumor cells was obtained at a ratio of stimulator tumor cells to responder lymphocytes of 1 : 1 or 1 : 5. No positive blastogenic response was obtained at a ratio of 1 : 25.
3) The optimal time for maximum stimulation of peripheral lymphocytes by autochthonous tumor cells was found to be varied from 4 to 6 day culture in each case.
4) It was indicated a significant correlation between MLTR and non specific lymphocytes blastogenic responses induced by PHA in this group studied (r= 0.520, p＜0.01).
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