J.Jpn. Surg. Soc.. 79(5): 355-363, 1978

---

Original article

RENAL CRYOPRESERVATION (2). THE EFFECT OF DIMETHYLSULFOXIDE CONCENTRATION ON SURVIVAL OF CANINE KIDNEYS FROZEN TO -22℃ .

The canine kidneys were perfused with 800 ml cryoprecipitated plasm a containing various concentration of dimethylsulfoxide (7.5, 12.5, 15.0, and 17.5% ). They were frozen to -22°C at rates of 2.0° to 4.0°C/min and thawed rapidly (70° -110°C/min) by a microwave unit which frequency was 2450 MHz and power output 500W.
Equilibration and nearly uniform distribution of DMSO were found in renal tissues following perfused with 12.5% DMSO for 30 minutes. Fifteen and 17.5% DMSO in the perfusate resulted in relatively lower kidney conceration than 7.5 and 12.5%
Eleven out of fourteen kidneys perfused with 12.5% DMSO were able to support life after contral a teral nephrectomy. Average survival time was ten days, however, two of the dogs in this group were allowed to longterm survivors.
Three out of six kidneys perfused with 15.0% DMSO were viable following contral ateral nephrectomy. Average survival time was 5 days. Uremia caused the death in these dogs.
None of the kidneys perfused with 7.5 and 17.5% DMSO had renal function.
The minimum renal cell damage as assessed by LDH and GOT in the post-thaw perfusate were found in the groups of 12.5 and 15.0% DMSO.
There was a gradual decrease in vascular resistance during post-thaw perfusion in the kidneys perfused with 12.5 and 15.0% DMSO. However, vascular resistance was increased drastically after eight minutes in the kidneys perfused with 7.5 and 17.5% DMSO.
These results suggest that viable kidneys frozen to -22°C can be achieved with the optimal freezing rates of 2.0° to 4.0°C/min and rapidly thawed to 70° to 110°C/min after perfused with 12.5% DMSO for 30 minutes.

---

---

To read the PDF file you will need Adobe Reader installed on your computer.