J.Jpn. Surg. Soc.. 79(2): 93-110, 1978

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Original article

EXPERIMENTAL AND CLINICAL STUDIES ON THE "IN VITRO" TRANSFER OF THE ANTITUMOR ACTIVITY WITH TUMOR-IMMUNE RNA TO NORMAL LYMPHOCYTES

Macrophages obtained from rats and guinea pigs were incubated with Yoshida sarcoma (YS) antigens (RNA and 105000 xg sediment of YS), and then, RNA was extracted from these macrophages (YS-immune RNA).
When normal rat's lymphocytes were incubated with YS-immune RNA and transfered into YS-bearing rats intraperitoneally, 30-50% of YS-bearing rats survived the challenge with YS cells. Mixed lymphocytes and tumor cells cytotoxicity test (MLTC) with ³H-thymidine and macrophage migration inhibition test (MIT)
revealed the antitumor activity of the lymphocytes incubated with YS-immune RNA. No definite difference of antitumor activity between allogeneic and xenogeneic RNA was recognized.
In clinical studies, guinea pig macrophages were incubated with the 105000 xg sediment extracted from the resected each tumor tissue of 41 cases with various cancers, and RNA was extracted from these macrophages (tumor -immune RNA).
Lymphocytes obtained from healthy volunteers with the same blood type of each patient were incubated with the individual specific tumor-immune RNA, and the cytotoxicity test was examined with sensitized lymphocytes, respectively.
Sixty eight per cent of 41 cases revealed a positive cytotoxicity with MLTC and 63% was positive with MIT.
In the 50% of the positive cases of them, cytotoxic effect was disappeared by addition of patient's own serum.

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