J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 2028KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 78(10): 875-882, 1977

Original article

RENAL CRYOPRESERVATION. (1) . THE EFFECT OF FREEZING RATE AND WARMING RATE ON SURVIVAL OF CANINE KIDNEYS FROZEN TO −22℃.

First Department of Surgery St. Marianna university School of Medicine. (Director: Prof. Hiromu Watanabe, M. D.)

Sunao Kubota

The effect of freezing rate and warming rate upon survival of canine kidneys frozen to -22 ℃ was investigated. The canine kidney was perfused with 800 ml cryoprecipitated plasma containing 12.5 % dimethylsulfoxide (DMSO), methylprednisolone 10 mg/g of kidney weight, isoproterenol 1.0 mg, MgSo41.0 g, fluorophenylalanine 0.4 mg/g of kidney weight and heparin 1000 units, frozen to -22 ℃ at rates ranging from 0.1°to 10 ℃/min. and thawed rapidly (70°- 110 ℃/min.) or slowly (20°- 30 ℃/min.) with using an electromagnetic wave. Success or failure of this technique was dependant upon freezing rate and warming rate.
All 14 kidneys frozen at rates 2.0° to 4.0 ℃/min. and thawed rapidly produced urine immediately after reimplantation. Three kidneys became swollen and infarcted becouse of venous thrombosis. All the remaining had function and performed contralateral nephrectomy after 3 to 4 weeks reimplanation. There were two longterm survivors. One survived for 72 days and the other for over 2 years. The shortest was 5 days in 3 dogs. The mean survival time of 9 dogs except two long survivors was 10 days. Eight kidneys frozen at rates of below 1.0 ℃/min. and either rapidly or slowly warmed had function to produce small amount of urine, however, they did not survive more than 5 days after contralateral nephrectomy. None of the kidneys frozen at rate of 0.1°and 10 ℃/min. survived following reimplanation.
Renal cell damage assessed by measuring lactic dehydrogenase (LDH) and glutamic oxaloacetic transaminase (GOT) in IU/ml/g of kidney weight in the initial 20 ml renal venous effluent of post-thaw perfusion. The minimum renal cell damage was found in the 2.0°to 4.0 ℃/min freezing rate following rapid warming (LDH:5.7 ±0.6 IU/ml/g of kidney weight. GOT:1.7±0.2 I U/ml/g of kidney weight). The deleterious freezing rate was 0.1°and 10 ℃/min.
The results suggest that viable kidneys frozen to -22 ℃ can be achieved with the optimal freezing rates of 2.0°to 4.0 ℃/min and rapidly thawing rates of over 70 ℃/min.

To next page >>

To read the PDF file you will need Adobe Reader installed on your computer.