J.Jpn. Surg. Soc.. 78(4): 327-344, 1977

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Original article

CLINICAL AND EXPERIMENTAL STUDIES ON THE ALTERATION OF MUCOPOLYSACCHARIDE OF GALLBLADDER-MUCOSA WITH CHOLELITHIASIS

The auther have made a postulation that striking proliferation of the gallbladder mucosal epithelium result from the abnormal secretion and absorption of the mucopolysaccharide in the chronic and acute inflammation, and a correlative biochemical and electron microscopic study has been performed.
Biochemical quantitative study :
On 103 gallbladder removed from patients with cholelithiasis, the mucopolysaccharide which is contained in the gallbladder wall is extracted by method of Scott and Schiller. The content is analyzed by electrophoretic method with Cellulose Acetate Membrane and stained with Alcian Blue. The basic patterns involved in this electrophoresis consist of three fractions of acid mucopolysaccharide, that is, hyaluronic acid, heparitin monosulfuric acid and chondroitinsulfuric acid. In varied types of chronic and acute inflammation, however, the another peak appears which shows positive reaction to PAS staining as well as to alcian blue staining, and it is identified as sialomucopolysaccharide which contains sialic acid. The percentage of this fraction is significantly increased in proportion to the severity of the inflammation in comparison with the others.
Electron microscopic study :
In accordance with severity of inflammation, vigorous proliferation of the celluar organellae, such as microvilli of cell coat, coated vesicles, mitochondria and Golgi complexes, are observed. Particularly, mucin globules are significantly increased in mucin producing cells. Stain intensity of the above mentioned organellae to PAM and ruthenium red staining is well correlated with vigorous proliferation of mucin.

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