J.Jpn. Surg. Soc.. 78(4): 313-326, 1977

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Original article

EXPERIMENTAL PATHOLOGIC STUDIES OF PULMONARY VASCULAR INJURY INDUCED BY ENDOTOXIN ADMINISTRATION.
－WITH SPECIAL REFERENCE TO CORRELATION WITH PATHOGENESIS OF SHOCK LUNG

This study was undertaken to know how lead to vascular change and shock state in lung of the male Donryu rat following intra-abdominal injection of 20 mg per kg of E. coli 0-26 endotoxin. At the same time the vascular clearance method was used to elucidate morphological diffusion barrier and to clarify pathogenesis of pulmonary change. The rats were sacrificed chronologically at 30 minutes, 1, 3 and 5 the hours after endotoxin injection.
At the first 3 hours histological changes were engorgement, aggregation of polymorphonuclear leukocytes and vascular regressive change such as endothelial swelling, subendothelial edema and vacuolization of medial cells in bronchial and pulmonary arteries. These changes were extensive and associated with occasional hemorrhages and a telectatic lesions at 5 hours.
By electron microscopy, at 3 hours, increased pinocytotic vesicles, mitochondrial swelling and lysosomal dense bodies were observed in endothelium and medial cells of bronchial and pulmonary arteries. These changes were associated with dilatation of intercellular junction, vacuolization and disruption of polymorphonuclear leukocytes and platelets especially in microvasculatures. The aggregation and disintegration of polymorphonuclear leukocytes and platelets may be resulted from circulatory disturbance or the direct cytotoxic effect of the endotoxin.
In the vascular permeability, the extent of increased permeability depends upon the qualitative difference of properities of vesicles and vesicular transport system in the vascular structure.
The so-called shock lung may be induced by the above mentioned various vascular changes and increased permeability which is distinctly substantiated from the investigation in virtue of the tracer methology.

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