J.Jpn. Surg. Soc.. 62(3): 372-380, 1961
ANAEROBIC BACTERIOLOGICAL STUDIES OF APPENDICITIS
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Department of Surgery, Nippon Medical School (Chief: Prof. Saburo MATSUKURA) Hajime SAKAI |
(1) Experimental materials were obtained aseptically from the appendices at the time of operation
(2) The culture medium used for isolating the bacteria was a modification of the Weinberg method, utilizing Holl test tube and v.f. bouillon.
(3) The anaerobic bacteria isolated from the materials comprised 7 species 54 strains (58.6%), consisting of 5 strains of Staphylococcus anaerobicus, 6 strains of Micrococcus niger, 5 strains of Bacillus ramosus, 9 strains of Bacillus bifidus, 1 strain of streptobacillus, 4 strains of Bacillus welchii.
(4) Of the 7 species 54 strains as above isolated, there were 2 species 11 strains of cocci and 5 species 37 strains of bacilli, while 8 species of cocci were obtained from normal cases.
(5) The coccal species were more numerous in normal appendices, while in all types of acute appendicitis the bacillary species were predominant.
(6) Bacillus welchii was isolated most frequently, namaly, 24 strains (26%), which. however, was far below the percentage previously reported. This organism was isolated from many cases, but the highest percentage was found in gangrenous type and in a less number of cases of purulent type, although it was also isolated from normal cases.
(7) Pathogenicity was noted in Bacillus welchii, B. ramosus and B. funduliformis, the greatest toxicity having been found in B. welchii. In spite of the highest occurrence (24 strains), identified even in normal cases, no clinical symptoms characteristic of any disease type could be recognized as being exclusively due to Bacillus welchii, thus making it as the sole agent in the etiology of appendicitis.
(Author's abstract)
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