J.Jpn. Surg. Soc.. 60(5): 743-758, 1959

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MICROSCOPIC OBSERVATIONS ON THE BEHAVIOR OF LIVING BLOOD VESSEIS DURING THE ANTIGEN-ANTIBODY REACTION BY TRANSILLUMINATION
CHAPTER II. OBSERVATIONS ON CIRCULATING BLOOD IN THE LIVER

By using the method of transillumination, the normal blood circulation of the liver of the mouse and rat and the attitude of the liver at the time of antigen-antibody reaction was observed, the salient results thus obtained being recorded in photograph and motion picture.
The observation was carried out on the edg of the liver, and the blood was seen to flow smoothly in the order of the intrahepatic branches of the portal veins, sinusoid and branches of the hepatic veins, the distribution of veins being either in the form of twigs or bushes.
The main body of the blood flowing into the lobulus at the edge of the liver consisted of the intraheprtic branches of the portal veins, with hardly any hepatic arteries being observed. The hepatic blood capillary circulation was seen to be irregularly intermittent in some parts but it was never observed to stop in the part over 75%. It was not also considered to stop or commence the circulation in the whole area of a lobulus at a time.
When the rats sensitized by passive sensitization were administered with horse serum or egg white albumine, promotion of the portal pressure (30-40 mm H₂O) was seen in 15-20 sec. at the same time that the blood circulation of the liver was accelerated. Sometimes the blood flow would become sluggish or stop entirely. Although such was seen to be exceptional, death was brought about at times.
In the case of the antigen-antibody complex obtained by precipitation reaction in vitro, also, the same reaction as in the case of the injection of the antigen was observed.
It was also known that a function existed in the rabbit serum obtained from the portal veins during the appearance of the antigen-antibody reaction, which would accelerate the blood flow.
While splenectomy was entirely unrelated to the change in the liver blood circulation caused by the antigen-antibody reaction, the change was especially manifest at both suprarenalectomy, leading to statis or death, showing a typical anaphylactic shock. In the case of a blocking of the reticuloendotherial system, the antigen-antibody reaction was nearly completely obstructed by two-time intravenous admin istration of India ink.
(author 's abstract)

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