J.Jpn. Surg. Soc.. 60(4): 679-695, 1959

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MICROSCOPIC OBSERVATIONS ON THE BEHAVIOR OF LIVING BLOOD VESSELS DURING THE ANTIGEN-ANTIBODY REACTION BY TRANSILLUMINATION
CHAPTER I. OBSERVATIONS ON CIRCULATING BLOOD IN THE MESENTERIC VESSELS OF THE RATS.

Using the method of transillumination specially designed by the present writer, observations were made principally on the attitude of the antigenantibody reaction in the mesenteric capillary circulation of the rats together on the change in the blood circulation at normal time and at time and at time of drugs taking effect, and the results thus obtained were recorded in photograph and motion picture.
Regarding the change brought in the blood circulation when administering various drugs of effective density, it was observed that contractibility was manifest in the case of adrenaline being applied and dilatibility in the cases of acetylcholine and histamine being given. In the cases of the administration of acetylcholine and histamine, moreover, promotion of the capillary permeability was observed, which, however, was noted to decrease with the administration of adrenaline. It was also observed that a repeated use of these drugs might produce a peculiarly contradictory change in the reaction. (Practised, paradoxical phenomenon).
When the antigen is applied to the mesentric surface sensitized by passive sensitization, a universal stasis was observed over the entire area of application, while intravenous administration of the antigen was seen to bring about the acceleration of blood flow. Sometimes, however, the blood flow would become sluggish or stop. Thus, it is seen that the same antigen-antibody reaction will take a different attitude according to the difference of method adopted for causing it.
In the experiment on the administration of the antigen, diapedese bleeding was seen at various spots after the appearance of stasis, such bleeding being observed not only in the venule but also in the true capillary.
The promotion of capillary permeability was even more manifest than at th time of the administration of acetylcholine and histamine.
All the positive group of the antigen-antibody reaction was seen to have a high sensitivity to acetylcholine, but no such tendency was observed in the case of histmine.
Fserine strongly stimulated the reaction in blood circulation by the antigen-antibody reaction, while chlorpromazine, on the contrary, obstructed it. Atropin decreased the reaction to a certain extent, but the antihistaminic substance neither obstructed nor promoted it.
Regarding the relation between the antigen-antibody reaction, it was observed tha more positive instanaces were seen in high-temperature summer-time and more negatve instances on low-temperature days.
(author's abstract)

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