J.Jpn. Surg. Soc.. 58(8): 1229-1238, 1957
BACTERIOLOGICAL STUDIES OF CHOLECYSTITIS
Ⅱ. RELATIONSHIP BETWEEN BACTERIA AND BILE ACIDS
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Department of Surgery, Nihon Medical School (Director: Prof. Saburo MATSUKURA) Tsugio TANAKA |
1. The inhibitory action of desoxycholic acid on the growth of bacteria was extremely remarkable. This effect was particularly noticeable against gram-positive cocci and anaerobic organisms, but it also exerted the same efect against Escherichia coli type I. which was relatively resistant, even in the low concentration of 10 mg%. A significant contrast was noted with cholic acid which exhibited a depressive effect somewhat markedly only against the cocci, the other strains such as Escherichia coli type I. and the anaerobes remaining unaffected even in a concentration as high as 50 mg%.
2. Concentration of the total amount of desoxycholic acid was extremely low in the bile of patients with cholecystitis when compared with the values found in the bile of normal individuals or of patients with duodenal ulcers. The value was also generally low in the bile of patients with gastric cancer, although in a few cases it was within the normal range. Positive demonstration ofbacteria in the bile was obtained only in those cases with the values above 20 mg% of total desoxycholic acid.
3. For the purpose of elucidating the mechanism involved in the depression of bacterial growth by desoxycholic acid, the effects of adding SH agents, para-amino benzoic acid and folic acid to the media were observed. It was found that desoxycholic acid exerted a seriously depressive influence on such vital systems as of SH enzyme and of chief metabolism as well as on the synthesis of nucleic acid. These effects were interpreted as being directly responsible for the inhibitory action of the acid on bacterial growth.
(author's abstract)
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