J.Jpn. Surg. Soc.. 57(12): 2072-2082, 1957
THE CRYSTALLIZATION OF GALL BLADDER BILE AND ITS CLINICAL USE FOR THE DETECTION OF GALL STONE
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1st Department of Surgery, Faculty of Medicine, Kyushu University,, Fukuoka City (Director: Prof. H. Miyake) Yoshio NISHIMURA |
The bladder bile obtained at operations directly from gall bladder was filtrated and allowed to dry on a watch glass in an incubator at 37°C for overnight. By this procedure, bladder bile removed from the gall bladder in patients of cholelithiasis always, with few exceptions, formed characteristic fern like structure or crystal nucleus. On the contrary, bladder bile obtained from cases of cholecystitis without gall stone formed amorphous substance or atypical crystals (thrum like structure, cross structure or plate). And the normal bladder bile didn't form any crystallization.
These crystals were identified as sodium chloride crystals by means of X-ray diffraction (Debye-Scherrer's method).
The chloride concentration in bladder bile was measured by electrical potentiometer and it was almost parallel to the degree of crystallization. The adding test of sodium chloride to normal bladder bile suggested that the bladder bile removed from the normal human being and cholecystitis without stone have the some factors of anticrystallization, though the bladder bile of cholelithiasis has not this factor.
Identical results were observed on so-called “B” bile obtained by Lyon's method (duodenal intubtion) following posterior pituitary extract (atonin) injection. And these results were checked on the bile obtained at operation.
This simple desication test might be a handy diagnostic procedure in the confirmation of the existence of gall stone.
(author's abstract)
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