J.Jpn. Surg. Soc.. 57(2): 159-174, 1956
A STUDY OF THE CHANGE PRODUCED BY HCL IN THE ISOELECTRIC POINT ON THE ENDOTHELIAL CELLS OF THE BLOOD CAPILLARIES OF THE DOG GASTRIC MUCOSA
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2nd Surgical Department, Kyushu University School of Medicine (Director: Prof. M. Tomoda) Shigemasa KOGA |
The author, with the object to gain some information about the action of HCl exert on the interstitial tissue of the gastric mucosa before it produces any microscopical hanges on the mucosa, measured by the staining method the isoelectric point on the endothelial cells of the blood capillaries in the interstitial tissue after infusion of HCI into the gastric lumen of the dog, and the above mentioned isoelectric point was compared with the isoelectric point that was measured after the intragastric infusion or ingestion of saline solution, mustard solution, or a certain food. The functioning of the surface epithelial cells over that part of the mucosal interstitial tissue in which HCl infused had caused acute inflammation, was also examined by determining the isoelectric point on the cells of the area. It was examined, besides, by the same method whether or not there was any local difference in the different parts of the pyloric glandular region in identical dog as to the function of the surface epithelium of the mucosa.
By the measurement of these isoelectric points the author concluded as follows:
1) Saline solution and food of any kind produced no change in the isoelectric point on the endothelial cells of the blood capillaries in the interstitial tissue of the dog gastric mucosa.
2) No change in the above-mentioned isoelectric point was produced by 500 cc of 0.3% HCl infused in 2.5 hours, or by 50 cc of 0.5% HCl infused in 2.5 hours.
3) The isoelectric point on the endothelial cells of the blood capillaries of the mucosal "Leiste" in the pyloric glandular area was shifted to the acid side 2.5 hours after infusion of 500 cc of 0.5% HCl and still more so 6 hours after that. That on the endothelial cells directly over the deep-lying lamina muscularis mucosae was also shifted to the acid side 6 hours after the infusion. This shift of the isoelectric point showed that the interstitial tissue of the mucosa was promoted in its function. In the fundic glandular area the isoelectric point only on the endothelial cells in''Leiste" was slightly to the acid side in 6 hours, showing that the interstitial tissue was slightly promoted in its function. The isoelectric point was generally shifted more to the alkaline side on the endothelial cells of the blood capillaries in the mucosa in both the fundic and pyloric glandular areas 12 hours after the infusion than it was 6 hours after that, showing the function of the interstitial tissue in the areas was gradually normalized in course of time.
4) In the view of the function of the interstitial tissue of the mucosa by the measurement of the isoelectric point, it was observed that there was a different sensibility to HCl between the fundic and pyloric glandular mucosa.
5) 5% mustard solution infused into the gastric lumen of the dog brought about a shift to the acid side of the isoelectric point on the endothelial cells of the blood capillaries in the fundic and pyloric glandular areas and caused a functional acceleration of the interstitial tissue of the mucosa. But, unlike the cases after the infusion of HCl, no local difference was observed in their sensibility to the HCl.
6) A shift to the alkaline side of the isoelectric point on the surface epithelial cells of the dog gastric mucosa in which HCl infused had produced inflammation of the interstitial tissue without causing necrosis of the surface epithelial cells was noted and its shift showed a functional change of them.
7) The isoelectric point was about equal in value on the surface epithelial cells in the different parts of the mucosa in identical pyloric glandular area, and the epithelial cells showed no functional difference in different parts. (author's abstract)
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