[Abstract] [Full Text PDF] (in Japanese / 717KB) [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 95(3): 179-186, 1994


Original article

SERUM β-N-ACETYL HEXOSAMINIDASE AS A NEW MARKER OF GRAFT VIABILITY IN CANINE ORTHOTOPIC LIVER TRANSPLANTATION

First Department of Surgery, Kobe University School of Medicine, Kobe, Japan

Masahiro Tominaga, Yonson Ku, Yoshikazu Kuroda

We investigated the correlation between graft viability and serum β-N-acetyl hexosaminidase (β-NAH), a well characterized circulatory lysozomal enzyme degraded specifically by the Kupffer cell. Orthotopic liver transplantation (OLT) was performed in 17 dogs; Group I, 1hr preservation and survival beyond 3 days; Group II, 1hr preservation and survival less than 2 days; Group III, 20 hr preservation. Serum β-NAH was compared with AST and TBA. Serum β-NAH (nmol/ml/hr) reached the peak values of 611±125,1227±310 and 3952±685 until 6 hr after reperfusion in groups I, II and III, respectively. AST (IU/L) reached the peak levels of 929±350, 1581±396 and 1945±394 at 24 hr in groups I, II and III, respectively. TBA (μmol/l) reached the peak levels at a time point immediately before reperfusion in group I (37.9±7.8) and II (42.5±8.4), whereas prolonged elevation was still continued even after reperfusion in group III. AST gave significant separation only after 24 hr (p<0.01) between groups I and II and at 15 min (p<0.01) between groups I and III. In contrast β-NAH and TBA showed slignificant separation between groups I and II at 4 hr (p<0.01) and between groups I and III at 15 min (p<0.01). These results suggest that serum β-NAH is an early, sensitive marker of graft function reflecting both hepatic cellular damage and functional recovery of the reticuloendothelial system.


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