J.Jpn. Surg. Soc.
ISSN 1880-1129

[Abstract] [Full Text PDF] （in Japanese / 3589KB） [Members Only And Two Factor Auth.]

J.Jpn. Surg. Soc.. 90(4): 573-579, 1989

Original article

ESTABLISHMENT OF AN INBRED STRAIN OF LEC(LONG EVANS CINNAMON) RATS WITH SPONTANEOUS HEPATITIS

Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan
*) The First Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan
**) Chromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo, Japan
***) Department of Pathology, Sapporo Medical College, Sapporo, Japan

Tsutomu Namieno, Noritoshi Takeichi, Kimimaro Dempo***), Michio Mori***), Junichi Uchino*), Motomichi Sasaki**), Hiroshi Kobayashi

At the Center for Experimental Plants and Animals, Hokkaido University, two inbred strains, Long Evans Cinnamon (LEC) and Long Evans Agouti (LEA), which were selected for coat colour, were isolated from a closed colony of Long Evans rats.
While the two inbred strains were maintained by sibmating, only LEC rats, over 24-generation, spontaneously developed acute hepatitis with sudden appearance of systemic jaundice at around four months after birth. The frequency of acute hepatitis was 80 to 90% and the disease in nearly 80% of these rats were progressive and they died within two weeks after onset, with their clinical course and histopathological findings similar to those of human fulminant hepatitis.
LEC rats with spontaneous hepatitis had strong-conversion of urine-bilirubin, ultimate increase of blood-bilirubin and abnormal increase of serum-transamynases (GOT, GPT; GOT>GPT). Histopathological findings of the Iivers in the rats with acute hepatitis showed spotty necrosis and abnormal hepatocytes containing giant bizarre nuclei and in the rats with fulminant-type hepatitis showed central or coagulated necrosis and marked infiltration of inflammatory cells.
Serum levels of albumin in LEC rats befor being affected by hepatitis were low compared with those of LEA rats and especially characteristic fact was that cellulose-acetate electrophoresis could not reveal gamma-globulin fraction in LEC rats of 6-week and 12-week old, which will be a clue to analyze the etiology of hepatitis in LEC rats.

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