J.Jpn. Surg. Soc.. 89(4): 508-515, 1988
Original article
A MONOCLONAL ANTIBODY, KM10 REACTIVE WITH HUMAN GASTROINTESTINAL CANCER
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First Department of Surgery, Kobe University, School of Medicine, Kobe, Japan Hidefumi Ishida, Harumasa Ohyanagi, Shuichi Okumura, Yoichi Saitoh |
The antibody reacted strongly with the plasma membrane of human gastro-intestinal carcinoma. Sections of both malignant and benign tissues were tested with immunoperoxidase. Ten of 10 (100％) large intestine cancers, 26 of 31 (84％) gastric cancers, 5 of 7 (71％) pancreatic cancers and 3 of 3 (100％) ampulla of Vater cancers reacted positively. Moderate or little reactivity was observed with normal human tissues and carcinomas of the liver, mammary, thyroid and adrenal glands.
According to a study of the distribution of 125I-labeled KM10 in nude mice bearing human gastric cancer, KM10 selectively localized in tumor tissue rather than normal tissue. Whole-body autoradiography also supPorted such selective distribution.
By enzyme treatment and Western blot analysis, the antigenic determinant of KM10 antigen was demonstrated to be protein with a MW of about 240,000.
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