J.Jpn. Surg. Soc.. 88(1): 109-118, 1987

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Original article

THE EFFECT OF PHARMACOLOGICAL PRETREATMENT ON THE LONG-TERM GLOBALLY ISCHEMIC HEART ; LYSOSOME, CYCLIC NUCLEOTIDE AND MYOCARDIAL INJURY

The effect of pretreatment on the 3 hrs globally ischemic heart assessed from the myocardial enzymes, lysosomal enzymes and cyclic nucleotides in coronary sinus blood was studied in mongrel dogs. Combined administration of CoQ₁₀, Aprotinin, Betamethasone and Nifedipine was done as pretreatment. Results : (1) m-AST, NAG and β-Glucuronidase were significantly lower in the pretreatment group. (2) Significant positive correlations were obtained in order between β-Glucuronidase and NAG (r＝0.6869) , m-AST and β-Glucuronidase (r＝0.6590), m-AST and NAG (r＝0.5381), m-AST and CK-MB (r＝0.49), respectively. (3) Significant increase in cAMP/cGMP ratio was observed in the control group after reperfusion. (4) Before aortic occlusion, significant negative correlations were obtained between cAMP andβ-Glucuronidase, NAG, m-AST, and after 5 min of reperfusion, significant negative correlations were obtained between cGMP and NAG, m-AST and significant positive correlations between cAMP/cGMP ratio and NAG, m-AST.
These data suggested the effectiveness of pretreatment, the relation between lysosomal enzyme release and the ischemic myocardial injury, and the usefulness of m-AST to evaluate the degree of myocardial injury. Moreover, the increase of cAMP/cGMP ratio suggested the ischemic myocardial injury, and cGMP in the ischemic condition, and cAMP in the non-ischemic condition affected lysosomal enzyme release.

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