J.Jpn. Surg. Soc.. 84(3): 196-202, 1983

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Original article

IMMUNOTHERAPY OF POSTOPERATIVE CANCER PATIENTS WITH IMMUNE RNA-SENSITIZED LYMPHOCYTES AND TUMOR ANTIGEN

Tumor cell suspension was prepared from resected tumor tissue of various cancer patients. I-RNA was extracted from lymphoid tissues of rabbits immunized with each tumor cells and CFA .
When the postoperative condition of patient recovered stable, autologous lymphocytes were prepared with blood cell separator (IBM 2997 type) and incubated with I-RNA, then, return to himself. Total number of sensitized lymphocytes was 4×10⁹－1.2×10¹⁰. After this passive immunization was finished, active immunization with autologous 3M KCl tumor extract was added. Three of gastrointestinal cancer, 3 of lung cancer, 2 of miscellaneous malignant tumor were treated with this schedule. T-cell ratio, blastogenesis of T-cell with PHA, skin reaction with PPD and PHA, CEA, IAP and Ig in serum was not defmitely changed before and after the immunotherapy. But 3 of them, LAI and LMI became to positive after the treatment and NK cell activity also increased in 3 cases.
In these 3 cases, tumor regression was recognized after the treatment. In a case of synovial sarcoma, skin reaction for autologous tumor antigen became to positive and the metastatic lesion in the lung was gradually regressed and regression is still continuing 6 months after the treatment (reduction ratio: 90%).

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