J.Jpn. Surg. Soc.. 83(5): 434-438, 1982

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Original article

HUMORALLY MEDIATED MYOCARDIAL HYPERREACTIVITY AND HYPOREACTIVITY IN EXPERIMENTAL ENDOTOXEMIA

The myocardial reactivity of dogs plasma, which was collected after administration of 1.75mg/kg E.coli endotoxin was studied in 8 dogs. Catecholamines, especially, epinephrine and norepinephrine in the plasma rose and reached to 2021±1027ng/ml and 1031±447ng/ml, respectively, 2 hours after endotoxin infusion.
The plasma was bathed in the chamber mounted with rat papillary muscle and was incubated with isolated rat myofibrills and mitochondria. Two hours after endotoxin infusion, the plasmas enhanced myofibril-ATPase activity and increased developed tension of the papillary muscle. There was a significant correlation between catecholamines and myosin-ATPase activity (r=0.62, p＜0.001).
On the other hand, mitochondrial succinate dehydrogenase activity and oxidative phosphorylation had remarkably decreased, during incubation with endotoxemic plasma. Five hours after endotoxin infusion, tension decreased significantly. Electronmicrograph demonstrated the desruption of inner and outer membranes of the mitochondria, and the derangement of actin and myosin filaments in swelled saps.
The myocardial reactivity after endotoxin infusion was also discussed in the text.

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