J.Jpn. Surg. Soc.. 83(5): 415-424, 1982
Original article
IN VIVO AND IN VITRO SENSITIVITY TEST OF HUMAN CANCERS SERIALLY TRANSPLANTED TO NUDE MICE
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Department of Surgery, School of Medicine, Keio University, Tokyo, Japan (Director :Prof. Osahiko Abe) Yuji Hanatani |
Five stomach carcinomas, 2 colon carcinomas, 1 breast carcinoma and 1 soft tissue sarcoma, serially transplanted subcutaneously to BALB/c nude mice were used for the experiment. Mitomycin C, aclacinomycin A, adriamycin and cyclophosphamide were studied. Because cyclophosphamide is inactive in vitro, 4-hydroperoxy-cyclophosphamide was used for in vitro study.
In vivo sensitivity tests were conducted in accordance with the Battelle Columbus Laboratories Protocol. For in vitro study, single cell suspension of cancer cells were prepared by trypsinization. The culture conditions were as follows ; the number of cancer cells was 2×104/well, incubation time was 48 hours and duration of labelling with 3H-thymidine was 24 hours. Evaluation was performed based on the rate of 3H-thymidine incorporation by cancer cells.
As to mitomycin C and cyclophosphamide, statistically significant correlations (r=0.731 and 0.713, respectively) were demonstrated between the results of both sensitivity tests. Therefore it was suggested that the results of in vitro sensitivity tests of cancer cells to mitomycin C and cyclophosphamide may provide an index to predict the effect of the drugs in vivo in the treatment of human solid cancers.
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